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Representative images were acquired using a Deltavision microscopy and Deltavision deconvolution software package (Applied Precision, Deltavision).

Confocal microscopy {#Sec16}
——————-

HeLa cells were cultured in glass bottom dishes (MatTek) and maintained in DMEM (Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum, 2 mM [l]{.smallcaps}-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin (all Invitrogen) at 37°C and 5% CO~2~ in a humidified incubator. Prior to imaging, cells were pre-incubated with complete medium containing 5 μM of the membrane impermeable nuclear dye Hoechst 33342 (Invitrogen) for 15 min. For imaging the cells, the laser for visualization of the GFP was excited at 488 nm and emissions between 450 and 500 nm were collected. For imaging the Hoechst 33342, we excited the laser at 405 nm and emissions between 430 and 500 nm were collected. Confocal images were generated with a Zeiss LSM 510 META and the software ZEN 2009.
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